Improving carotenoid pigments production in Rhodotorula mucilaginosa using UV irradiation

ثبت نشده
چکیده

Rhodotorula yeasts were isolated from various local sources in Damascus, Syria, during 2012 and 2013. Fifty isolates (23 from leaves trees, 13 from different soils, 6 from different meats, 6 from dairy products, 1 pickle and 1 traditional sweet) were identified using API 20C AUX system. Isolated yeasts were classified into three Rhodotorula species: R. mucilaginosa, R. glutinis and R. minuta representing 76%, 20% and 4% of total isolates tested respectively. All isolates were able to produce carotenoid pigments that varied in their quantities ranging from 13.61 μg/g dry biomass for isolate A28 to 658.23 μg/g for isolate A23. The wild strain A23 of R. mucilaginosa was subjected to mutagenesis using UV irradiation at a wavelength of 254 nm. Highest carotenoid production within isolated mutants reached a value of 734.58 μg/g dry biomass after an irradiation period of 4.5 minutes. Overall, UV irradiation

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

منابع مشابه

Evaluation of the Relationship between the Incubation Time and Carotenoid Production in Rhodotorula Slooffiae and R. Mucilaginosa Isolated from Leather Tanning Wastewater

  Objective(s): Carotenoids which are naturally synthesized by fungi such as yeasts can act as an antioxidant which is closely related to their ability to decrease the risk of a variety of degenerative diseases. In recent years, the increase of demand for carotenoids obtained from natural sources has promoted major efforts to improve carotenoid production from biological sources such...

متن کامل

Evaluation of the Relationship between the Incubation Time and Carotenoid Production in Rhodotorula Slooffiae and R. Mucilaginosa Isolated from Leather Tanning Wastewater 

OBJECTIVE(S) Carotenoids which are naturally synthesized by fungi such as yeasts can act as an antioxidant which is closely related to their ability to decrease the risk of a variety of degenerative diseases. In recent years, the increase of demand for carotenoids obtained from natural sources has promoted major efforts to improve carotenoid production from biological sources such as pigmented ...

متن کامل

Whole genome sequencing of Rhodotorula mucilaginosa isolated from the chewing stick (Distemonanthus benthamianus): insights into Rhodotorula phylogeny, mitogenome dynamics and carotenoid biosynthesis

In industry, the yeast Rhodotorula mucilaginosa is commonly used for the production of carotenoids. The production of carotenoids is important because they are used as natural colorants in food and some carotenoids are precursors of retinol (vitamin A). However, the identification and molecular characterization of the carotenoid pathway/s in species belonging to the genus Rhodotorula is scarce ...

متن کامل

Evaluation of Antioxidant Activity of R. Slooffiae, R. Mucilaginosa Extracts

INTRODUCTION Antioxidants are health beneficial compounds that can protect cells and macromolecules from the damage of reactive oxygen species (ROS). The aims of this study were to compare the total antioxidant and carotenoid production in R. Slooffiae and R. Mucilaginosa. METHODS To isolate the carotenoid pigment, cells were suspended in acetone and broken using a homogenizer, followed by ce...

متن کامل

Statistical optimisation of cell growth and carotenoid production by rhodotorula mucilaginosa

Sequential statistical methods were used to maximise carotenoid production by a strain of Rhodotorula mucilaginosa, isolated from the Brazilian ecosystem. Initially, a factorial 2(5-1) experimental design was used, and the variables were pH and the levels of glucose, yeast extract, MgSO4.7H2O and KH2PO4. The nitrogen source (yeast extract) was the most important variable in enhancing carotenoid...

متن کامل

ذخیره در منابع من


  با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

عنوان ژورنال:

دوره   شماره 

صفحات  -

تاریخ انتشار 2016